Rate of Absorbance in Catechol

Observing the Rate of Production in an Enzyme (Catecholase) Catalyzed Reaction and flyer the Absorbance of Light at 70 degrees Celsius Alicia Lopez Abstract Write at the end Introduction The map of my themes look into is to measure the rate of deed of the enzyme found in potatoes. The enzyme is called Catecholase. When I combined potato rip with catechol, the chemical reaction that occurs is called benzoquinone. quinone is the reddish brown gouge found in potatoes. My groups hypothesis is if that if we conjure the temperature of the enzyme and the potato extract wherefore the reaction rates ordain be considerably higher(prenominal) and more light leave be absorbed when measured. The heat variable will modify the protein. In the essay, I raise the temperature of the Catechol and the enzyme from room temperature to 70 degrees Celsius separately. I then combined the heated extract with the catechol and measured the total of light that was absorbed by the product, benzoquinone. I timed it in 20 second increments and noted the result in a spreadsheet. Materials and Methods Our group consist of myself, Lydia Huber, Erin and Ashley. The experiment was conducted in Cell biota for Health Lab single out on July 22, 2009.

For comparison purposes and experience, forward to the experiment we conducted a connatural experiment without the heating variable. This overly allows us to become well-known(prenominal) with a machine familiar called the spectrophotometer. It measures the transparency of an object or outcome by emit a beam of light through and through it.! The product that is founder during the reaction absorbs light. We set the mode to contagion and the wavelength to 540nm as instructed by the instruction sheet provided by our instructor. The initial thing we did is to make sure we had all the necessary tools and chemicals needed. The chemicals consisted of a bottle of distilled water, 50 mL potato extract, 50 mL of 0.05%...If you want to reduce a full essay, order it on our website: BestEssayCheap.com

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